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. 2016 Jul 26;9(8):e35052. doi: 10.5812/jjm.35052

Table 2. PCR Program for the Studied Genes.

Factor Temperature, °C
Gene mecA ant(4’)-Ia aac(6’)-Ie/aph(2”) aph(3’)-IIIa
Primary denaturation 94 94 94 94
Denaturation 94 94 94 94
Annealing 57 47 45 51
Extension 72 72 72 72
Final extension 72 72 72 72