Table 2. PCR Program for the Studied Genes.
Factor | Temperature, °C | |||
---|---|---|---|---|
Gene | mecA | ant(4’)-Ia | aac(6’)-Ie/aph(2”) | aph(3’)-IIIa |
Primary denaturation | 94 | 94 | 94 | 94 |
Denaturation | 94 | 94 | 94 | 94 |
Annealing | 57 | 47 | 45 | 51 |
Extension | 72 | 72 | 72 | 72 |
Final extension | 72 | 72 | 72 | 72 |