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. 2016 Oct;57(13):5558–5567. doi: 10.1167/iovs.16-19965

Figure 7.

Figure 7

Oxidative stress-related gene and protein expression after FCD-LIRD. RNA was isolated from eyes without light damage (n = 8 eyes), 4 hours after “Light-only” (30 minutes of 125 K lux) FCD-LIRD (n = 6 eyes) and 24 hours after “Light-only” FCD-LIRD (n = 4 eyes). Values for ΔCT (CtPROBE − CtGAPDH) are shown for eyes collected at 4 (A) or 24 (B) hours after light exposure. A smaller bar indicates a higher level of gene expression. Gene expression changes were determined using the formula: RQ = 2 − ΔΔCt (Supplementary Tables S3, S4). Immunohistochemistry of retinal sections from naïve (C, E) or light exposed (D, F) C57BL/6J mice demonstrates increased HO-1 (D) and C3d (F) staining after light damage using 2 mg of intraperitoneal fluorescein followed 10 minutes later by 4 minutes of illumination with 54 K lux of white light (“Fl-4@10”). Scale bar: (D) 75 μm.