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. 2016 Oct;57(13):5525–5534. doi: 10.1167/iovs.16-19691

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Thy-1 is expressed in CECs and upregulated by AMD-related stresses. (A) Western blot of Thy-1 protein in cultured HRPE (top panel) and in CECs before passaging (p0) and after in vitro culturing (passages 2 [p2] and p5) (bottom panel); Western blot of Thy-1 protein (top panel) and qPCR of Thy-1 mRNA (bottom panel) (B) in CECs treated with CCL11 (10 ng/mL) or VEGF (20 ng/mL) overnight (*P < 0.05 vs. PBS, n = 6) and (C) in RPE/choroids isolated from young (20- to 40-year-old) and old (>60-year-old) donor eyes (***P < 0.001 vs. Young, n = 3).