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. 2016 Oct;57(13):5525–5534. doi: 10.1167/iovs.16-19691

Figure 4.

Figure 4

Knockdown of Thy-1 inhibits VEGF-mediated Rac1 activation and angiogenic effects in CECs. (A) Rac1 activity assay, (B) CEC migration, (C) CEC proliferation, and (D, E) cytoskeleton stress fibers stained by rhodamine phalloidin (D, representative images; E, quantification of fluorescent density of stress fiber staining [normalized to control siRNA/PBS]) in CECs transfected with Thy-1 siRNA and treated with VEGF (20 ng/mL) for 30 minutes. Note intracellular fibers induced by VEGF and reduction in Thy-1 siRNA-transfected CECs compared to control siRNA (*P < 0.05, **P < 0.01, ***P < 0.001 vs. PBS of control siRNA; ††P < 0.01, †††P < 0.001 vs. VEGF of control siRNA; ‡P < 0.05 vs. PBS of Thy-1 siRNA; n = 6–9).