Fig. 1.

Reporter system for the detection of proteins containing ncAAs on the yeast surface. (A) Yeast transformed with reporter (single chain variable fragment-Fc) and suppression constructs produce full-length proteins when induced in media containing a ncAA recognized by the orthogonal aaRS. (B) Flow cytometry analysis of reporter protein display levels detected by labeling the cmyc and HA epitope tags. Prior to detection, cultures were induced in media containing 1 mM p-azido-L-phenylalanine (AzF; 1), 1 mM p-acetyl-L-phenylalanine (AcF; 2), or no ncAA.