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Biophysical characterization of FNfn10-α-lys and FN3con-α-lys. CD thermal melts of (A) FNfn10-α-lys (Tm of 43 ± 2°C) and (B) FN3con-α-lys (Tm of 101 ± 3°C). SEC, revealing complex formation for (C) FNfn10-α-lys and (D) FN3con-α-lys with lysozyme. Representative BLI sensograms of (E) FNfn10-α-lys and (F) FN3con-α-lys with titrations of the respective FN3 protein against an immobilized HEL surface (fit for each concentration as a thin black line).
