Figure 3.

Overexpression of gigaxonin clears neuronal IF aggregation in GAN KO neurons. DRG neurons were transduced with lentiviruses that express either FLAG-tagged gigaxonin or GFP (as a control). There was a significant reduction in the levels of all subtypes of neuronal IF: NFL (A), NFM (B), NFH (C), α-internexin (D) and peripherin (E). Note that when cells expressed different levels of gigaxonin, then the cell with a greater expression of gigaxonin (asterisks) show decreased levels of the neuronal IF, as shown in B, D and E. Scale bar: 10 µm.