Figure 2.

5hmC in umbilical veins preferentially resided in intragenic regions, and reduced on gene bodies under the conditions of GDM and preeclampsia. (A) Umbilical vascular 5hmC preferred intragenic regions. Annotation of umbilical vascular 5hmC peaks from the control, GDM and preeclampsia revealed their enrichment in intragenic features such as exons and promoters. Log2-fold changes over expected values were calculated. Features enriched by 5hmC were indicated by color bars, and depleted were indicated by grey bars. (B) 5hmC specifically reduced on refseq gene bodies in the umbilical veins from GDM and preeclampsia conditions. Normalized 5hmC read densities were calculated as reads per million on known hg 19 refseq gene bodies (n = 52 291), and average reads were plotted in the control and disease conditions using ngsplot. Two kilobase flanking regions upstream and downstream of gene bodies were also included.