Fig. 10.

Percentage of cell death and nitrite levels in spinal cord injury slices. Viable cells, identified using 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, were visualized under light microscopy. The level of cell death was evaluated in each slice at 7 days. Cell death in injured groups was considerably higher compared with the control group. (a) Cell death was significantly attenuated by 18B12 pretreatment. ***p <0.001 vs control (CTR); ^### p < 0.001 vs damage. (b) Indeed, an augmented formation of nitrite levels was observed in the damage group, while the pretreatment with 18B12 decreased injury-induced nitric oxide (NO) production. ^*** p < 0.001 vs CTR; ^# p < 0.1 vs damage