Fig. 9.

Effects of 18B12 treatment on regulatory T cell (Treg) expression. (l, o) Spinal cord sections from sham-operated mice expressed basal levels for forkhead box P3 transcription factor (Foxp3). 18B12 treatment reduced the degree of positive staining for (n, o) Foxp3 with respect to (m, o) spinal cord injury (SCI) mice. The figure is representative of at least 3 experiments performed on different experimental days. Data are expressed as percentage of total tissue area. Data are mean ± SEM of 10 mice from each group.**p < 0.01 vs sham. ^## p < 0.01 vs SCI. Also, spinal cord sections were double stained with antibodies against CD4 (green) and Foxp3 (red). (b, e) Increased Treg cell expression was revealed in SCI mice, (c, f) while Foxp3 and CD4 immunoreactivity was reduced in 18B12-treated mice.Yellow arrows indicate CD4 and Foxp3 expression