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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Chem Res Toxicol. 2016 Feb 22;29(3):303–316. doi: 10.1021/acs.chemrestox.5b00468

Figure 2.

Figure 2

Polymerase-DNA affinity. Fluorescence anisotropy was determined with 1 nM DNA in which the 5′-terminus of the primer strand was modified with fluorescein with variable concentrations of (A) pol η, (B) pol ι, and (C) pol κ. The DNA contained dT (open circle), O2-Me-dT (black circle), O2–POB-dT (square). The data points are the mean ± standard deviation of three determinations. The lines are the best fit to eq 3.