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Pol ζ catalyzed extension of O2-alkyl-dT base pairs. The relative Vmax/Km values for dA, dC, dG, and dT as at the primer terminus (X) with dT, O2-Me-dT, and O2-POB-dT as base pair partner (Y). The steady-state kinetics were performed with 30 nM DNA (P16/T24) and 1 μg/μL pol ζ. The data points are the mean ± SD of three independent determinations.
