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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Chem Res Toxicol. 2016 Feb 22;29(3):303–316. doi: 10.1021/acs.chemrestox.5b00468

Table 2.

Steady-State Parameters for the Pol η Catalyzed Insertion Opposite O2-alkyl-dTa

dNTP template kcat (min−1) Km (μM) kcat/Km (mM−1 min−1)
dATP dT 49 ± 2 5.3 ± 0.3 9230 ± 230
dTTP dT 0.140 ± 0.004 77.8 ± 5.5 1.8 ± 0.1
dATP O2-Me-dT 0.068 ± 0.003 18.5 ± 2.4 3.7 ± 0.4
dCTP O2-Me-dT 0.068 ± 0.003 130.2 ± 11.4 0.52 ± 0.02
dGTP O2-Me-dT 0.039 ± 0.001 38.4 ± 1.8 1.05 ± 0.04
dTTP O2-Me-dT 0.119 ± 0.004 166 ± 9 0.72 ± 0.02
dATP O2-POB-dT 0.098 ± 0.004 21.7 ± 2.5 4.50 ± 0.37
dCTP O2-POB-dT 0.141 ± 0.008 148.6 ± 16.2 0.95 ± 0.05
dGTP O2-POB-dT 0.121 ± 0.004 126.2 ± 8.0 0.96 ± 0.03
dTTP O2-POB-dT 0.126 ± 0.005 103.3 ± 8.6 1.22 ± 0.06
a

Initial rates were conducted with 0.25 to 0.5 nM pol, 5 nM DNA, and 0 to 200 μM dNTP. The values are the mean ± standard deviation of three determinations.