Table 2.
dNTP | template | kcat (min−1) | Km (μM) | kcat/Km (mM−1 min−1) |
---|---|---|---|---|
dATP | dT | 49 ± 2 | 5.3 ± 0.3 | 9230 ± 230 |
dTTP | dT | 0.140 ± 0.004 | 77.8 ± 5.5 | 1.8 ± 0.1 |
dATP | O2-Me-dT | 0.068 ± 0.003 | 18.5 ± 2.4 | 3.7 ± 0.4 |
dCTP | O2-Me-dT | 0.068 ± 0.003 | 130.2 ± 11.4 | 0.52 ± 0.02 |
dGTP | O2-Me-dT | 0.039 ± 0.001 | 38.4 ± 1.8 | 1.05 ± 0.04 |
dTTP | O2-Me-dT | 0.119 ± 0.004 | 166 ± 9 | 0.72 ± 0.02 |
dATP | O2-POB-dT | 0.098 ± 0.004 | 21.7 ± 2.5 | 4.50 ± 0.37 |
dCTP | O2-POB-dT | 0.141 ± 0.008 | 148.6 ± 16.2 | 0.95 ± 0.05 |
dGTP | O2-POB-dT | 0.121 ± 0.004 | 126.2 ± 8.0 | 0.96 ± 0.03 |
dTTP | O2-POB-dT | 0.126 ± 0.005 | 103.3 ± 8.6 | 1.22 ± 0.06 |
Initial rates were conducted with 0.25 to 0.5 nM pol, 5 nM DNA, and 0 to 200 μM dNTP. The values are the mean ± standard deviation of three determinations.