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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Chem Res Toxicol. 2016 Feb 22;29(3):303–316. doi: 10.1021/acs.chemrestox.5b00468

Table 3.

Steady-State Parameters for the Pol ι Catalyzed Insertion Opposite O2-alkyl-dTa

dNTP template kcat (min−1) Km (μM) kcat/Km (mM−1 min−1)
dATP dT 8.0 ± 0.6 2.6 ± 0.4 3070 ± 270
dTTP dT 0.103 ± 0.003 70 ± 4 1.46 ± 0.06
dATP O2-Me-dT 0.076 ± 0.002 24 ± 1 3.2 ± 0.1
dCTP O2-Me-dT 0.061 ± 0.004 93 ± 14 0.65 ± 0.06
dGTP O2-Me-dT 0.114 ± 0.002 46 ± 2 2.5 ± 0.1
dTTP O2-Me-dT 0.121 ± 0.005 42 ± 5 2.9 ± 0.2
dATP O2-POB-dT 0.086 ± 0.003 35 ± 3 2.4 ± 0.1
dCTP O2-POB-dT 0.059 ± 0.003 112 ± 13 0.53 ± 0.03
dGTP O2-POB-dT 0.094 ± 0.003 28 ± 3 3.3 ± 0.2
dTTP O2-POB-dT 0.121 ± 0.001 37 ± 1 3.27 ± 0.08
a

Initial rates were conducted with 0.25 to 0.5 nM pol, 5 nM DNA, and 0 to 200 μM dNTP. The values are the mean ± standard deviation of three determinations.