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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Chem Res Toxicol. 2016 Feb 22;29(3):303–316. doi: 10.1021/acs.chemrestox.5b00468

Table 4.

Steady-State Parameters for the Pol κ Catalyzed Insertion Opposite O2-alkyl-dTa

dNTP template kcat (min−1) Km(μM) kcat/Km
dATP T 4.3 ± 0. 2 1.4 ± 0.1 3200 ± 200
dTTP T 0.11 ± 0.01 120 ± 20 1.0 ± 0.1
dATP O2-Me-dT 0.087 ± 0.008 35 ± 8 2.5 ± 0.3
dCTP O2-Me-dT 0.067 ± 0.006 160 ± 30 0.41 ± 0.03
dGTP O2-Me-dT 0.055 ± 0.004 64 ± 12 0.86 ± 0.10
dTTP O2-Me-dT 0.067 ± 0.001 34 ± 2 1.99 ± 0.07
dATP O2-POB-dT 0.047 ± 0.001 33 ± 2 1.41 ± 0.05
dCTP O2-POB-dT 0.022 ± 0.002 29 ± 7 0.81 ± 0.16
dGTP O2-POB-dT 0.13 ± 0.01 66 ± 12 2.0 ± 0.2
dTTP O2-POB-dT 0.14 ± 0.03 215 ± 79 0.64 ± 0.10
a

Initial rates were conducted with 0.25 to 0.5 nM pol, 5 nM DNA, and 0 to 200 μM dNTP. The values are the mean ± standard deviation of three determinations.