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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Chem Res Toxicol. 2016 Feb 22;29(3):303–316. doi: 10.1021/acs.chemrestox.5b00468

Table 6.

Kinetic Parameters for the dNTP Dependence of the Pol ι Catalyzed Insertion Opposite O2-alkyl-dTa

template dNTP amplitude
burst rate constant
steady-state rate constant
Amax/[pol] KA (μM) Amax/[pol]/KA (mM−1) kpol (s−1) Kd dNTP (μM) kpol/Kd (s−1 mM−1) kssmax (s−1) Kss (μM)
dT dATP 3.0 ± 0.4 22 ± 6 0.25 ± 0.03 10 ± 5
O2-Me-dT dATP 0.420 ± 0.047 190 ± 60 8.9 3.3 ± 0.3 0.027 ± 0.267 78 ± 37
O2-Me-dT dCTP 0.180 ± 0.020 601 ± 130 0.3 2.3 ± 0.6 0.013 ± 0.003 250 ± 160
O2-Me-dT dGTP 0.093 ± 0.007 213 ± 56 0.4 5.5 ± 1.1 217 ± 121 25 0.017 ± 0.006 592 ± 426
O2-Me-dT dTTP 0.380 ± 0.033 104 ± 34 3.6 3.6 ± 0.2 0.023 ± 0.003 110 ± 40
O2-POB-dT dATP 0.313 ± 0.020 90 ± 20 3.5 3.7 ± 0.2 43 ± 12 86 0.017 ± 0.001 80 ± 20
O2-POB-dT dCTP 0.187 ± 0.013 300 ± 53 0.6 2.53 ± 0.05 27 ± 3 94 0.004 ± 0.001 8 ± 15
O2-POB-dT dGTP 0.147 ± 0.013 421 ± 105 0.3 2.42 ± 0.24 45 ± 22 54 0.007 ± 0.001 186 ± 63
O2-POB-dT dTTP 0.307 ± 0.033 82 ± 31 3.7 3.7 ± 0.3 42 ± 18 88 0.024 ± 0.004 170 ± 90
a

Reactions were conducted with 150 nM pol, 15 nM DNA, and 0 to 1000 μM dNTP. The individual time courses were fit to eq 2 to obtain amplitude (A), burst rate constants (kb), and steady-state rate constants (kss) as a function of dNTP concentration. These values were fit to the hyperbolic equation to obtain the parameters in the table. The values are the mean ± standard error of three determinations. If the K values are missing, then the parameter was not dependent on the [dNTP].