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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Chem Res Toxicol. 2016 Feb 22;29(3):303–316. doi: 10.1021/acs.chemrestox.5b00468

Table 7.

Kinetic Parameters for the dNTP Dependence of the Pol ζ Catalyzed Insertion Opposite O2-alkyl-dTa

template amplitude
burst rate constant
steady-state rate constant
dNTP Amax/[pol] KA (μM) Amax/[pol]/KA (mM−1) kpol (s−1) Kd dNTP (μM) kpol/Kd (s−1 mM−1) kssmax (s−1) Kss (μM)
dT dATP 0.3 ± 0.01 62 ± 2 8 ± 1 7750 0.86 ± 0.15 14 ± 5
O2-Me-dT dATP 0.49 ± 0.11 340 ± 190 1.4 3.3 ± 0.5 0.033 ± 0.005 202 ± 84
O2-Me-dT dCTP 0.18 ± 0.01 320 ± 27 0.6 3.2 ± 0.7 0.013 ± 0.003 438 ± 215
O2-Me-dT dGTP 0.14 ± 0.015 195 ± 21 0.7 4.8 ± 0.6 210 ± 80 22 0.010 ± 0.001 152 ± 30
O2-Me-dT dTTP 0.87 ± 0.47 1120 ± 960 0.7 3.2 ± 0.5 0.039 ± 0.006 364 ± 139
O2-POB-dT dATP 0.29 ± 0.03 85 ± 37 3.4 3.3 ± 0.3 20 ± 7 165 0.029 ± 0.006 260 ± 150
O2-POB-dT dCTP 0.25 ± 0.07 860 ± 410 0.3 2.9 ± 0.4 60 ± 40 48 0.014 ± 0.002 490 ± 170
O2-POB-dT dGTP 0.80 ± 0.67 5200 ± 4800 0.2 2.3 ± 0.1 10 ± 4 230 0.011 ± 0.002 380 ± 200
O2-POB-dT dTTP 0.51 ± 0.03 350 ± 60 1.5 2.8 ± 0.3 0.027 ± 0.004 110. ± 50
a

Reactions were conducted with 150 nM pol, 15 nM DNA, and 0 to 1000 μM dNTP. The individual time courses were fit to eq 2 to obtainamplitude (A), burst rate constants (kb), and steady-state rate constants (kss) as a function of dNTP concentration. These values were fit to the hyperbolic equation to obtain the parameters in the table. The values are the mean ± standard error of three determinations. If the K values are missing, then the parameter was not dependent on the [dNTP].