Table 8.

Steady-State Kinetic Parameters for Pol ζ Catalyzed the Extension Past O²-alkyl-dT^a

Y	Z	dNTP	X	Vmax nmol s−1 g−1	Km μM	Vmax/Km μ L/(s g)
A	T	A	dT	0.0674 ± 0.0067	20.9 ± 2.4	3.22 ± 0.16
A	T	C	dT	0.0472 ± 0.0047	35.7 ± 8.2	1.35 ± 0.21
A	T	G	dT	0.0674 ± 0.0040	25.2 ± 1.3	2.68 ± 0.28
A	T	T	dT	0.0678 ± 0.0090	23.4 ± 6.5	2.98 ± 0.40
T	A	A	O2-Me-dT	0.0421 ± 0.0078	38 ± 18	1.28 ± 0.52
T	A	C	O2-Me-dT	0.0411 ± 0.011	60 ± 31	0.73 ± 0.16
T	A	G	O2-Me-dT	0.0424 ± 0.00035	46 ± 14	0.96 ± 0.21
T	A	T	O2-Me-dT	0.0444 ± 0.0103	48 ± 26	1.03 ± 0.28
T	A	A	O2-POB-dT	0.0431 ± 0.0075	44 ± 16	1.02 ± 0.25
T	A	C	O2-POB-dT	0.0401 ± 0.0048	66 ± 16	0.62 ± 0.08
T	A	G	O2-POB-dT	0.0416 ± 0.0013	51 ± 11	0.83 ± 0.17
T	A	T	O2-POB-dT	0.0371 ± 0.0038	36 ± 11	1.06 ± 0.24

^aData are expressed as the mean ± SD of three independent experiments. The DNA substrate was P16/T24 as in Chart 1.