Skip to main content
. 2016 Oct 26;12(10):e1005984. doi: 10.1371/journal.ppat.1005984

Fig 10. Effect of Talin-1 on intimate attachment of C. rodentium.

Fig 10

(A) Tln1 mRNA level in YAMC cells transduced with Ctrl, Slc7a2, or Tln1 shRNA, infected or not with C. rodentium (C. rod). **P < 0.01; n = 3 independent experiments. (B) Effect of L-Lys on Tln1 expression in YAMC cells. ***P < 0.001; n = 3 independent experiments. (C) Cells were L-Arg-depleted or not and treated with actinomycin D (ActD); total RNA was extracted at the indicated times and Tln1 mRNA was analyzed by real-time PCR. The combined results of two independent experiments are shown and depicted as the percent of the Tln1 expression compared to the level before addition of actinomycin D. (D-E) YAMC cells were transduced with Ctrl or Tln1 shRNA infected with C. rodentium; FAS test (D) and Cxcl1 and Cxcl2 mRNA levels (E) were analyzed after 4 h. *P < 0.05, **P < 0.01; representative images of 3 independent experiments are shown. Scale bar, 20 μm.