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. 2016 Oct 26;11(10):e0165150. doi: 10.1371/journal.pone.0165150

Fig 3. TG-induced apoptosis in RPE cells and protection by HN.

Fig 3

Confluent hRPE cells were treated with varying concentrations (0.5–3 μM) of TG for 12 hours. (A) Percentage of TUNEL positive cells increased in a dose-dependent manner with TG treatment. (B) Representative images of TUNEL positive cells (red) and nuclei (blue) are shown for each experimental condition. (C) Pre-incubation with HN for 12 hours protected RPE cells dose-dependently from apoptosis induced by TG (3 μM). (D) Representative images are shown for each HN treatment group. Data are mean ± SEM (n = 3). Asterisks represent **p<0.01). Scale bar: 20 μm in B and D.