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. 2016 Oct 26;12(10):e1006400. doi: 10.1371/journal.pgen.1006400

Fig 3. Overexpression defects of Zasp52 transgenes are dosage-dependent.

Fig 3

(A) Western Blot visualizing UAS-Zasp52-PRΔPDZ driven by Mef2-Gal4 or UH3-Gal4. α-tubulin serves as loading control. (B) Confocal microscopy of IFM stained with anti-Flag antibody visualizing the transgene in green, anti-α-actinin antibody to label Z-discs in purple, as well as phalloidin to visualize actin thin filaments in red. Weak UH3-Gal4-mediated expression of UAS-Zasp52-PRΔPDZ reduces overexpression defects. Scale bar, 10 μm.