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. 2016 Oct 26;12(10):e1006400. doi: 10.1371/journal.pgen.1006400

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© 2016 Liao et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — (A) Cartoon of Zasp52 genomic locus with selected transcripts, the open reading frame with protein domains and three MiMIC lines shown. (B) Immunoblot of thorax extracts from wild type and the MiMIC lines Zasp52^MI07547, Zasp52^MI00979, and Zasp52^MI02988 incubated with anti-Zasp52 antibody. α-tubulin is used as a loading control. (C) Immunoblot of thorax extracts from wild type and the homozygous MiMIC line Zasp52^MI02988 incubated with anti-Zasp52 antibody. Longer exposure and loading more extract compared to wild type shows the presence of residual Zasp52 (arrows). α-tubulin is used as a loading control. (D) Lethality of homozygous Zasp52^MI02988 at different developmental stages.