Fig 7. Zasp52^MI02988 exhibits strong IFM defects.

Confocal microscopy of IFM of MiMIC lines transheterozygous over the deficiency Df(2R)BSC427 stained with phalloidin to visualize actin thin filaments in green and anti-Kettin antibody to visualize Z-discs in red. (A) Zasp52^MI02988 phenotypes range from wavy and frayed myofibrils to the separation of thin filaments from Z-discs, leaving actin-free gaps in the Z-disc area, to the complete disappearance of Z-discs and M-lines. (B) Zasp52^MI07547 IFM are indistinguishable from wild type. (C) Zasp52^MI00979 myofibrils are wavy and sometimes frayed, Z-discs and H-zones are often bent. Scale bar, 10 μm.