Fig 6. In vitro endothelial cell permeability, using condition media from ex vivo sorted Ly6C^+high monocytes culture.

(A) VEGF production was significantly increased in sorted Ly6C^+high monocytes at 6 and 12 hr. Values represent the mean ± SD (n = 4). *p <0.001, compared with the control media at 0 hr. (B) Ly6C^+high monocytes conditioned media harvested from a 6-hr culture increased endothelial cell permeability, as indicated by the increased streptavidin-HRP in the lower chamber. Values represent the mean ± SD (n = 4). ^#p <0.05 and *p <0.01, compared with the control or between groups.