Table 14A.3.1.
Cell Lines Used in Specific Vaccinia Protocols
| Cell line | Usea | Procedure |
|---|---|---|
| HeLa S3 | Virus stock preparation | UNIT 14A.3 Basic Protocol 3 |
| Virus purification | UNIT 14A.3 Basic Protocol 6 | |
| BS-C-1 | Plaque assay | UNIT 14A.3 Support Protocol 1 |
| Transfection | UNIT 14A.4 Basic Protocol 1 | |
| Plaque size screening | UNIT 14A.3 Basic Protocol 2 | |
| Fluorescent protein screening | UNIT 14A.4 Basic Protocol 2 | |
| XGPRT selection | UNIT 14A.4 Basic Protocol 2 | |
| Plaque amplification | UNIT 14A.4 Basic Protocol 3 | |
| HuTK− 143B | TK selection | UNIT 14A.4 Basic Protocol 2 |
| Plaque assay (optional) | UNIT 14A.3 Support Protocol 1 | |
| Transfection (optional) | UNIT 14A.4 Basic Protocol 2 | |
| CEF | MVA procedures |
UNIT 14A.3 Basic Protocol 5, Support Protocol 3; UNIT 14A.4 |
| Basic Protocol 4 | ||
| BHK-21 | MVA procedures |
UNIT 16.16 Basic Protocol 5, Support Protocol 3; UNIT 16.17 |
| Basic Protocol 4 |
a
The preferred use(s) for each cell line is listed first; if optional is indicated, the cell line can be used for the indicated procedure, but the results may not be as good as those from the preferred cell line.