Figure 3.

CTGF is a target gene of miR-145. (A) Binding of miR-145 with CTGF 3′-UTR predicted by TargetScan; (B) Dual luciferase reporter gene assay revealed that miR-145 significantly decreased the luciferase activity of CTGF wild-type (wt) 3′UTR; (C) CTGF mRNA levels in Eca109 cells transfected with miR-145 mimics, miR-145 inhibitor or scramble sequence were examined by qRT-PCR; (D) CTGF proteins were detected by Western blotting using GAPDH as a loading control. The results were from 3 experiments. Data are expressed as mean±SD. ** P<0.01 vs. corresponding control; * P<0.05 vs. corresponding control.