Effect of ω-3 diet on gene expression of immune cell markers and cytokine expression. A. mRNA expression level of markers of general macrophages (F4/80), M1 (iNOS) and M2 (ARG1, CD206, CD204) macrophages were determined using real-time qPCR relative to GAPDH mRNA. B. mRNA expression level of cytokines associated with general macrophages (CCL2), M1 (IL-6, TNF-α) and M2 (IL-10) was determined using real-time qPCR relative to GAPDH mRNA. n=15 for ω-6 diet group and n=14 for ω-3 diet group. Data are presented as the mean ±SEM; Student's t-test was used to compared ω-3 diet with the ω-6 diet (*, p<0.05).