Extended Data Figure 3. Unilateral photoinhibition of ALM immediately before movement causes ipsilateral bias.

a. Unilateral photoinhibition of ALM during different task epochs. Sample epoch, 1.3s; delay epoch, 1.3s. Photoinhibition, 0.5 s (0.4s and 0.1s ramp, Methods).

b. Performance with 0.5s photoinhibition of left or right ALM during different trial epochs. Performance was plotted as a function of time interval between photoinhibition offset (the end of ramp offset) and the onset of ‘go’ cue (T_recovery). Performance was not significantly affected for T_recovery>0.3s. Thick lines, mean; thin lines, individual mice (n = 5). *p<0.05, **p<0.01, ***p < 0.001, two-tailed t-test.

c. Unilateral photoinhibition of ALM during different task epochs. Sample epoch, 1.3s; delay epoch, variable duration, 1.2s – 1.7s in 0.1s increments. Trials with different delay epoch durations were randomly interleaved. Photoinhibition was for 1.3s (1.2s and 0.1s ramp, Methods), resulting in different T_recovery.

d. Performance with 1.3s photoinhibition. Plot is similar to (b). Performance was not significantly affected for T_recovery > 0.3s.

e. Photoinhibition (0.5 s) immediately before the ‘go’ cue is similar to the behavioral effect caused by photoinhibition during the entire delay epoch (1.3 s). Photoinhibition data at T_recovery=0 from (b) and (d) was re-plotted.