Figure 2.

GVS effect on GBM CSC sphere formation. (A) GBM1, GBM3, GBM5, GBM7, and GBM9 were maintained in the selective medium for stem cells in the absence or presence of increasing concentrations of GVS. Sphere formation was visually monitored and after 14 days the number of spheres/well was counted. Representative microphotographs (magnification 10X) are reported. (B) Histogram reports the mean sphere number from four experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ANOVA followed by Dunnett's post-hoc test).