Figure 5.

Modulation of autophagic marker expression following GVS administration in GBM1, GBM2, GBM3, GBM6, GBM7, and GBM9 CSCs. (A) LC3-I, LC3-II, Beclin1 and acetyl-α-tubulin protein levels was determined by immunoblotting. β-actin was used as protein loading control. Lysates were obtained from cells treated for 72 h with GVS (0.5, 1.0, and 2.0 μM) or rapamycin (50 nM). (B) Densitometric analysis of protein expression. Values were normalized to β-actin expression and reported as percentage of untreated controls. Experiments were performed in triplicate, data represent mean ± SEM, (*p < 0.05; **p < 0.01, ***p < 0.001, ANOVA followed by Dunnett's post-hoc test).