Figure 3.

HSV1 miR H8 Inhibits NK Killing

(A) HSV1 miR H8- and control miRNA-expressing BJAB cells labeled with ³⁵S methionine were co-cultured for 5 hr with the NK cell line YTS-ECO at the effector-to-target (E:T) ratios indicated.

(B) Primary bulk NK cells were incubated for 5 hr with BJAB cells expressing miR H8, a control miR, shPIGT, or a control shScramble labeled using ³⁵S methionine as above.

(C) BJAB cells expressing miR H8 were co-transduced to overexpress exogenous PIGT (PIGT OE) or an empty vector (ds-empty). These cells and control miR-expressing cells were used in NK killing assays as in (B).

Representative experiments were repeated twice for (A) and (C) and four times for (B). Target killing is displayed as a percentage of the total. Shown are mean values ± SEM. ^∗p > 0.05, ^∗∗p > 0.01, ^∗∗∗p > 0.005.