Figure 2.

The Demethylase Activity of Lsd1 Is Required to Maintain BAT Properties

(A and B) Macroscopic view (A) and H&E staining (B) of representative sections of BAT of mice treated with vehicle or Lsd1(i).

(C and D) Relative mRNA levels of WAT-selective (C) and BAT-selective (D) genes in BAT of mice treated with vehicle or Lsd1(i) (mean + SEM, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001; vehicle, n = 6; Lsd1(i), n = 7).

(E) Western blot analysis of Lsd1, Ucp1, and Apoe in BAT of mice treated with vehicle or Lsd1(i). β-Tubulin was used as a loading control.

(F) Oil red O staining of differentiated primary brown adipocytes treated with vehicle or Lsd1(i).

(G) Pie chart depicting BAT- and WAT-selective genes with Lsd1 peaks at promoters in differentiated primary brown adipocytes.

(H) HOMER motif analysis of Lsd1 ChIP-seq data unraveled Rest and Nrf1 binding sites among the top-scoring motifs.

Scale bars, 5 mm (A), 100 μm (B), 50 μm (F). See also Figure S2.