Figure 5.

Lsd1 Limits Fat Accumulation in BAT

(A) Metabolomic analysis of BAT of control and Lsd1^cKO mice.

(B) Lipidomic analysis of BAT from 10-week-old control and Lsd1^cKO mice.

(C) Relative mRNA levels of the indicated enzymes involved in fatty acid elongation in BAT of control and Lsd1^cKO mice (mean + SEM; ^∗∗p < 0.01, ^∗∗∗p < 0.001; Ctrl, n = 9; Lsd1^cKO, n = 7).

(D–F) Determination of fatty acid synthase (Fasn), lipase, and Lpl activities in BAT of Ctrl and Lsd1^cKO mice at 10 weeks of age (mean + SEM, ^∗∗p < 0.01, ^∗∗∗p < 0.001, n = 6).

(G) Mitochondrial respiration of BAT extracts from Ctrl and Lsd1^cKO mice assessed with a high-resolution respiratory Oxygraph-2K system using palmitoyl-L-carnitine as substrate (mean + SEM, ^∗∗∗p < 0.001, n = 6).

See also Figure S5 and Tables S4 and S5.