Figure 2.

EspG Interaction with Arf GTPase Blocks the WRC

(A) Cartoon depicting key residues (white) in EspG (black) responsible for interaction with Rabs (green), PAK (blue), or Arf (purple).

(B) WRC-dependent actin-based motility via membrane-anchored Arf1^QL and Rac1^QL (depicted in cartoon) alone (control) or in the presence of EspG, namely, wild-type (WT) or mutants in binding Rab (EspGΔ^R), PAK (EspGΔ^P) or both Arf and PAK (EspGΔ^AΔ^P).

(C) Interaction of EspG derivatives with membrane-anchored Arf1^QL in buffer.

(D) Proteins recruited by membrane-anchored Arf1^QL and Rac1^QL (colored circles) in the presence or absence of EspG derivatives as (B) analyzed by SDS-PAGE. Molecular weight markers in kilodaltons (left).

(E) Immunoblotting of samples from (D) with indicated antibodies (right).

(F) Phagocytosis of EPECΔ^espG (ΔespG) expressing a control vector or the vector encoding espG variants described in (B).

Error bars represent ± SEM. Asterisks indicate a significant difference from control (black bars). ns, not significant. See also Figure S2.