Figure 4.

Molecular Basis of WRC Interference by EspG

(A) Cartoon depicting Arf1 interaction with EspG or GAT3 of GGA3 with key residues and domains in Arf1 shown.

(B) WRC-dependent actin-based motility directed by Arf1^QL and Rac1^QL in extract alone (control), or with GAT3 or EspG. Insets magnify actin-comet tails. Scale bars 5 μm.

(C) Immunoblotting of proteins recruited by membrane-anchored Arf1^QL and Rac1^QL from extract alone (ctrl), or from extract containing EspG or GAT3, or both in combination. Anti-GST antibodies detected GAT3 and EspG.

(D) Interaction of EspG with PCPI membranes alone (–) or with membranes anchored with Arf1^QL (ctrl) or Arf1^QL derivatives containing indicated mutations.

(E) Experiment performed as (B) with GAT3.

See also Figure S3.