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. 2016 Oct 26;12(10):883. doi: 10.15252/msb.20167144

Figure 1. Determination of cell counts using the histone abundance for normalization.

Figure 1

  1. The core histones and overview of the corresponding QPrEST and peptide standards mapped out on the protein sequence.
  2. Relative quantification of all four histone proteins in each tissue replicate (order of appearance per replicate: H2A, H2B, H3.3, and H4).
  3. Immunohistochemistry images from the Human Protein Atlas (http://www.proteinatlas.org) for protein ANXA1 with nuclear staining (blue) for three selected tissues (scale bars = 100 μm).
  4. Calibration curves for two of the four histone peptides, with decreasing amount of QPrEST standard spiked into a U2OS cell lysate.