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. Author manuscript; available in PMC: 2017 Jun 6.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Apr 25;55(24):6882–6886. doi: 10.1002/anie.201601693

Table 1.

Detected muropeptides from whole-cell analysis (in molecules per bacterium ×104).a

Muropeptide Wild-type Inducedb p-valuec
NAG-1,6-anhNAM
2a 1.5 ± 0.07 0.3 ± 0.01 0.01*
2b 0.2 ± 0.01 0.08 ± 0.01 0.004*
2c 0.8 ± 0.05 0.3 ± 0.02 0.02*
2d 5.5 ± 0.3 1.0 ± 0.3 0.002*
2e 0.01 ± 0.005 0.1 ± 0.02 0.04*
1,6-anhNAM
4c 0.3 ± 0.06 0.1 ± 0.003 0.0003*
4d 0.4 ± 0.01 0.07 ± 0.003 0.01*
4e N.D.d 0.08 ± 0.01 0.04*
(NAG-NAM)n-NAG-1,6-anhNAM
3cc 0.1 ± 0.01 0.02 ± 0.006 0.003*
3cd 0.2 ± 0.02 0.03 ± 0.007 0.03*
3dd 0.9 ± 0.06 0.09 ± 0.03 0.002*
3ddd 0.1 ± 0.02 0.01 ± 0.003 0.049*
NAG-1,6-NAM-crosslinked-1,6-NAM-NAG
5cd 0.03 ± 0.004 0.02 ± 0.001 0.2
5dd 0.3 ± 0.01 0.07 ± 0.007 0.001*
6dd 0.04 ± 0.001 0.02 ± 0.004 0.07
Reduced end
2a-R 0.07 ± 0.01 N.D.d 0.02*
2c-R 0.07 ± 0.03 0.02 ± 0.002 0.11
2d-R 0.5 ± 0.1 0.1 ± 0.03 0.06
5dd-RR 0.1 ± 0.002 0.01 ± 0.002 0.0002*
5dd-R 0.02 ± 0.002 0.009± 0.002 0.047*
Totale 11.1 ± 0.8 2.4 ± 0.5
a

Average of two runs.

b

PAO1 was exposed to cefoxitin at 512 μg/mL.

c

p-values from Student t-test.

Numbers with *are significantly different (p < 0.05) between the wild-type and induced sample.

d

not detected.

e

total-detected muropeptides.