Table 1.
Muropeptide | Wild-type | Inducedb | p-valuec |
---|---|---|---|
NAG-1,6-anhNAM | |||
2a | 1.5 ± 0.07 | 0.3 ± 0.01 | 0.01* |
2b | 0.2 ± 0.01 | 0.08 ± 0.01 | 0.004* |
2c | 0.8 ± 0.05 | 0.3 ± 0.02 | 0.02* |
2d | 5.5 ± 0.3 | 1.0 ± 0.3 | 0.002* |
2e | 0.01 ± 0.005 | 0.1 ± 0.02 | 0.04* |
1,6-anhNAM | |||
4c | 0.3 ± 0.06 | 0.1 ± 0.003 | 0.0003* |
4d | 0.4 ± 0.01 | 0.07 ± 0.003 | 0.01* |
4e | N.D.d | 0.08 ± 0.01 | 0.04* |
(NAG-NAM)n-NAG-1,6-anhNAM | |||
3cc | 0.1 ± 0.01 | 0.02 ± 0.006 | 0.003* |
3cd | 0.2 ± 0.02 | 0.03 ± 0.007 | 0.03* |
3dd | 0.9 ± 0.06 | 0.09 ± 0.03 | 0.002* |
3ddd | 0.1 ± 0.02 | 0.01 ± 0.003 | 0.049* |
NAG-1,6-NAM-crosslinked-1,6-NAM-NAG | |||
5cd | 0.03 ± 0.004 | 0.02 ± 0.001 | 0.2 |
5dd | 0.3 ± 0.01 | 0.07 ± 0.007 | 0.001* |
6dd | 0.04 ± 0.001 | 0.02 ± 0.004 | 0.07 |
Reduced end | |||
2a-R | 0.07 ± 0.01 | N.D.d | 0.02* |
2c-R | 0.07 ± 0.03 | 0.02 ± 0.002 | 0.11 |
2d-R | 0.5 ± 0.1 | 0.1 ± 0.03 | 0.06 |
5dd-RR | 0.1 ± 0.002 | 0.01 ± 0.002 | 0.0002* |
5dd-R | 0.02 ± 0.002 | 0.009± 0.002 | 0.047* |
Totale | 11.1 ± 0.8 | 2.4 ± 0.5 |
Average of two runs.
PAO1 was exposed to cefoxitin at 512 μg/mL.
p-values from Student t-test.
Numbers with *are significantly different (p < 0.05) between the wild-type and induced sample.
not detected.
total-detected muropeptides.