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. 2016 Oct 27;6:35928. doi: 10.1038/srep35928

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Copyright © 2016, The Author(s)

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(A) Size evaluation of silica beads by nanosizer based on dynamic light scattering. (B) Design of a lower EV gate by hs-FCM based on acquisition of silica beads of various sizes (100 to 1000 nm). (C) Portrayal of non-activated platelets (PLT). (D) Remnant platelets detected in the EV fraction (after stimulation) using a cell counter and FCM (<2%). ND: not detected. (E) EVs from preparations cleared of any remnant platelets by centrifugations were analyzed by hs-FCM. Representative of 3 different donors. (F) Portrayal of RBC analyzed by FCM. (G) Remnant RBCs detected in the EV fraction (after stimulation) using cell counter and FCM (<16%). ND: not detected. (H) EVs from preparations cleared of any remnant RBCs by centrifugations were analyzed by hs-FCM. Representative of 3 different donors, *P < 0.05.