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. 2016 Oct 27;6:35928. doi: 10.1038/srep35928

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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After stimulation of platelets with thrombin, cell frequency decrease (blue) in platelets (1) and increase (red) in platelet EVs (9–10). Red blood cell markers (CD235a) (2,3), other cells (1), and noise/background (5) were mostly unchanged. Platelet CD41a⁺ EVs (9,10) and extracellular mitochondria lacking CD41a⁺ (h, naked mitochondria) were induced (red). Data are representative of 3 independent experiments.