Figure 6. Comparison of large-scale chromosome features.

(A) The upper triangle of the map of average spatial distance between 100 kb regions on chromosome 19 at the end of the steering dynamics is shown. The gray bands mark the centromeric region. The boundaries of the 13 spatial macrodomains, identified with a clustering analysis of the distance matrix (see Methods), are overlaid on the map and the boundaries of the spatial blocks from ref. 25 are shown below. The consistency of the two partitions is visually conveyed in the chromosome cartoon at the bottom. Overlapping regions, shown in blue, account for 63% of the chromosome (centromere excluded). (B) The RMSD of our chromosome models and the models inferred using the method in ref. 36 are shown for non-steered (red) and steered conformations using target contacts based on ref. 11 (blue). The similarity of the two models is very clearly increased by the constrained steering procedure, and particularly so for chromosomes with a denser set of constraints (chr19, chr17, chr16, and chr7).