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. 2016 Oct 27;6:35794. doi: 10.1038/srep35794

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Sanger sequencing analysis of PAH_c.1222C>T COS-7 cells by IRES reverse primer showed “A” point mutation on the PAH gene. (b) RT-PCR confirmed PAH_c.1222C>T expression in established cell line. (c) Western blot showed that the expression of PAH in PAH_c.1222C>T cells was lower than in PAH_WT cells.