Figure 6. Tumorsphere and derivative formation demonstrates the HemSC tumorigenic and differentiation potential.

(a) Tumorsphere controls. HUVEC were used as a negative control and glioblastoma CSC as a positive control. Glioblastoma CSC formed tumorspheres (lower), whereas HUVEC formed no tumorspheres (upper). (b) Derivative formation. HemSC-derived tumorspheres were grown as a suspension (in non-adherent ultra-low cluster plates) and then transferred onto an adherent matrix. The latter condition afforded the generation of differentiated derivatives that attached to and spread on the culture substrate. Bioimaging of HemSC-derived tumorsphere formation in non-adherent ultra-low cluster plates showed no derivative formation.