Figure 5. Impaired survival of T cells in T-HOIP^Δlinear mice.

(a) Thymocytes and spleen cells were stained with anti-CD4 and anti-CD8 antibodies together with Annexin V and 7AAD. CD4⁺ or CD8⁺ T cells with an Annexin V⁺7AAD⁻ phenotype were evaluated. The number indicates the percentage of each population. (b) Isolated CD4⁺ or CD8⁺ T cells from HOIP^+/+ or T-HOIP^Δlinear mice were cultured in the absence or presence of plate-coated anti-CD3 mAb. The cell number after the indicated number of days was counted. The value is calculated from the number of T-HOIP^Δlinear/number of HOIP^+/+ cells. *p < 0.05. (c) Isolated CD4⁺ T cells from HOIP^+/+ (CD45.2) or T-HOIP^Δlinear mice (CD45.2) were transferred into nonirradiated B6 mice (CD45.1). The number of donor cells 7 days after transfer was counted. Data are shown as means ± SEM. *p < 0.05. The data in these figures are representative of five independent experiments.