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. 2016 Oct 27;6:36102. doi: 10.1038/srep36102

Figure 1. Synergism between NKBRPa and SLα/β in stimulation of SLα gene expression.

Figure 1

(a) Time course of NKBRPa (1 μM), SLα (30 nM), and NKBRPa (1 μM)+ SLα (30 nM) on SLα mRNA expression in carp pituitary cells. (b) Synergistic effect of SLα/β with NKBa and NKBRPa in stimulation of SLα mRNA expression. In this experiment, carp pituitary cells were incubated for 24-hr with SLα/β (30 nM) co-treatment with NKBa, NKBRPa, and senktide (1 μM). (c) Effect of SLα concentration (0.01–100 nM) on basal and NKBRPa (1 μM)-induced SLα mRNA expression in carp pituitary cells. (d) Effects of NKBRPa concentration (0.1–1000 nM) on basal and SLα(30 nM)-induced SLα transcript levels in carp pituitary cells. (e) Synergistic effect of senktide (1 μM) with SLα and SLβ in stimulation of SLα mRNA expression. Receptor specificity for SLα regulation by SLα/β with NKBRPa. In this experiment, carp pituitary cells were challenged for 24-hr with SLα (30 nM)+ NKBRPa (1 μM) or SLβ (30 nM)+ NKBRPa (1 μM) in the presence or absence of NK3R antagonist SB222200 (10 μM). After drug treatment, total RNA was isolated for real-time PCR of SLα mRNA. In the data present (mean ± SEM), the groups denoted by different letters represent a significant difference at p < 0.05 (ANOVA followed by Dunnett’s test).