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. 2016 Oct 27;6:36102. doi: 10.1038/srep36102

Figure 2. Up-regulation of NK3R gene expression by SLα/β in grass carp pituitary cells.

Figure 2

(a) Time course of carp SLα (30 nM) and SLβ (30 nM) treatment on NK3R mRNA expression in carp pituitary cells. (b) 24-hr incubation with increasing levels of SLα or SLβ (0.01–100 nM) treatment on NK3R mRNA expression in carp pituitary cells. (c) Effects of 24-hr co-treatment with the PI3K inhibitor Ly294002(10 μM) and wortmannin (1 μM), Akt inhibitor HIMOC (10 μM) and mTOR inhibitor rapamycin (20 nM) on SLα (30 nM)- or SLβ (30 nM)-induced NK3R transcript expression in carp pituitary cells. (d) Effects of 24-hr co-treatment with the JAK2 inhibitor HEX (50 μM), STAT5 inhibitor IQDMA (50 μM), MEK1/2 inhibitor U0126 (10 μM) and p38 MAPK inhibitor PD169816 (10 μM) on SLα (30 nM)- or SLβ (30 nM)-induced NK3R mRNA expression. After drug treatment, total RNA was isolated for real-time PCR of NK3R mRNA. In the data present (Mean ± SEM), the groups denoted by different letters represent a significant difference at p < 0.05 (ANOVA followed by Dunnett’s test).