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Schematics of NP-EGF preparation. 80 nm Au NPs are functionalized with PEG1 (=HS-EG77-N3) and PEG2 (=HS-(CH2)11-EG6-COOH) to introduce binding sites and stabilize the NPs, respectively. Functionalized human EGF is then covalently linked to PEG1 through the CuI catalyzed 1,3-dipolar cycloaddition reaction.
