Table 1. Perturbations on hydrogenase enzyme characteristics by the antibody interactions.
| Immobilized scFv | H2 production (nmol)a |
P(T <= t) two-tailb | |
|---|---|---|---|
| Set 1 (After anaerobic wash step) | Set 2 (After aerobic wash step) | ||
| 7Ac | 188 ± 43 | 141 ± 19 | 0.181 |
| 59Ac | 168 ± 52 | 150 ± 37 | 0.649 |
| 82Ac | 255 ± 24 | 116 ± 55 | 0.027 |
| 48In | 239 ± 25 | 66 ± 42 | 0.025 |
| Bead control | 14 ± 7 | 0 | ND |
| Enzyme control | 1041 ± 145 | 0 | 0.006 |
aH2 evolution activity of streptavidin-scFv-antigen complexes exposed to aerobic and anaerobic buffers by methyl viologen oxidation assay. The H2 production and standard deviations presented are mean data from 3 independent assays.
bThe Two-Sample t-Test Assuming Unequal Variances (p value) was performed using the H2 production (nmol) data from bead immobilized scFv-hydrogenase complex in Set 1 (anaerobic wash) to the corresponding sample in Set 2 (aerobic wash).
ND, Not determined.