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Inhibition of Wnt/β-catenin signaling does not lead to spontaneous differentiation in ELF1 hESCs. (A) Representative bright field images of ELF1 hESCs after they were cultured on MEF for 7 d in the indicated small molecules. Yellow arrow indicates spontaneous differentiation. (Scale bar, 100 µm.) (B) Representative flow cytometry plots of the Tra1-60+/CD9+ population of ELF1 hESCs treated as described in A of two biological replicates.
