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. 2016 Oct 3;113(42):E6382–E6390. doi: 10.1073/pnas.1613849113

Fig. S9.

Fig. S9.

Wnt inhibition leads to primed-like changes in naïve hESCs. (A) Results of qRT-PCR–based analysis of CRABP2 expression in ELF1-2iLIF and H1-4iLIF hESCs grown on Matrigel and treated with the indicated small molecules for 3 d. Fold changes were normalized to the value in ELF1-2iLIF-DMSO. For ELF1-2iLIF hESCs, data represent mean + SEM of n = 2 biological replicates. For H1-4iLIF hESCs, data represent mean + SEM of n = 3 biological replicates (*P < 0.05 by t test). (B) Plot of OCR measured using the Seahorse assay in H1-4iLIF hESCs grown on Matrigel with the indicated small molecules for 3 d. The data are the mean ± SEM of n = 5 technical replicates. The plot is representative of n = 3 biological replicates. Ant/Rot, Antimycin and Rotenone; FCCP, carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone; Oligo, oligomycin.