Fig. 3.

Tumor-killing effect of TPZ in HCC following transient L-HAL in the HBx transgenic mice model. The mice were treated with 0.9% saline (n = 2), doxorubicin (10 mg/kg, n = 2), or TPZ (3 mg/kg, n = 3) through tail vein injections before transient L-HAL and were killed on day 7. (A) Representative gross morphology of the liver and tumors. Tumor necrosis occurred in the HCC treated with TPZ, and transient HAL was revealed as a pale color. The blue and green dashed cycles indicate the tumor regions with and without HAL treatment, respectively. (B) Representative histopathological examination of the TPZ-treated mice. Nearly complete coagulative necrosis was observed in the left lobe HCC after treatment with the L-HAL and TPZ, but not in the HCC in the right lobe or the nontumor region. (C) No necrotic changes were detected in HCC from the groups treated with the combination of transient HAL and saline or doxorubicin. The data are representative of each independent group. (Scale bars: 0.2 mm.)